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Fig. 4. (A) The effect of inhibitors of phosphoinositide 3-kinase on PAF-induced Ca2+ transients in two-cell embryos. The results are the mean and s.e.m. of at least 30 embryos from three replicates. Embryos were treated with PAF:acetylhydrolase, then with the inhibitors and concentrations shown and then challenged with 37 nM PAF/ml in the presence of inhibitors. (B) The expression of phosphoinositide 3-kinase subunits in the two-cell embryo. A: mRNA for P110 catalytic subunits in two-cell embryos and brain tissue. M is molecular weight size markers (GeneRuler DNA Ladder Mix). Lane 1 lacked reverse transcriptase; Lane 2, no template acted as a negative controls; Lane 3, ß-actin (243 bp; positive control); Lane 4, p110{alpha} (238 bp); Lane 5, p 110ß (146 bp); Lane 6, p110g (111 bp); and Lane 7, p110{delta} (bp 294). B: mRNA for P85 regulatory subunits in two-cell embryos. M is molecular weight size markers. Lane 1, no reverse transcriptase; Lane 2, no template; Lane 3, two-cell ß-actin; Lane 4, P85 {alpha}-subunit (128bp), Lane 8 P85, ß-subunit (159 bp).