Fig. 6. Cenp-F is not required for kinetochore localization of Bub1, BubR1 or Mad2. DLD-1 or DLD-1 Myc-Mad2 cells were transfected with control or Cenp-F siRNA duplexes, treated with nocodazole and then fixed and stained to detect Cenp-F (blue), centromeres/kinetochores (ACA, green), DNA and Bub1, BubR1, Myc-Mad2 or Cenp-E (red). (A) Representative projected image stacks showing that repression of Cenp-F reduces kinetochore localization of Cenp-E. Scale bar, 5 µm. (B) Normalized pixel intensities in control (black bar) and Cenp-F-repressed (white bar) cells. Values represent the mean and s.e.m. of at least 30 kinetochores in three different cells.