Fig. 5. p130Cas silencing by siRNAs inhibits estrogen-dependent Erk1/2 MAPK activation. T47D cells were transfected with p130Cas siRNAs (+) or with control siRNAs (–) as described in Materials and Methods. 48 hours after transfection cells were stimulated with 10 nM estrogen for different times and detergent extracted. Cell extracts were run on 8% SDS-PAGE and blotted with the indicated antibodies. The same results were obtained in two independent experiments.