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Fig. 5. T. brucei procyclic cells at various phases of the cell duplication cycle. Phase-contrast images and corresponding immunofluorescence images; DNA is labelled with DAPI (blue), the flagellum is identified by L8C4, anti-PFR antibody (green) and the flagellar connector is labelled by AB1 antibody (red). (A) An AB1 signal is not seen in most 1K1N cells. (B) AB1 is observed in a proportion of cells just above the basal body in phase contrast. (C,D) The AB1 signal is seen in cells that are elongating a new flagellum at the correct position for the flagella connector and throughout the cell cycle. (E) The AB1 signal is not seen in cells that have entered cytokinesis. Scale bars, 2 µm