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Fig. 1. Immunoblot analysis of the changes in cell junction protein expression. MDCK cell lines expressing control neo gene (MDCK-neo), Snail (MDCK-Sna), E-cadherin (MDCK-Ecad), or Snail and E-cadherin together (MDCK-Sna-Ecad) were lysed in SDS sample buffer and subjected to immunoblot analysis. HA-tagged Snail protein and ectopically expressed myc-tagged mouse E-cadherin protein were detected with the indicated antibodies. Myc-tagged E-cadherin migrates more slowly than the endogenous canine E-cadherin. Vinculin serves as an internal control for protein loading.