JCS -- Fujita et al. 117 (9): 1719 Figure IG2

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Fig. 2. Association of ${alpha}$ B-crystallin with MTs. (A) Co-localization of ${alpha}$ B-crystallin and MTs in formaldehyde fixed L6E9 cells. (B) Co-localization of ${alpha}$ B-crystallin and MT in methanol fixed L6E9 cells. (A,B) Merged immunofluorescence micrographs (right) of the same cells visualized for ${alpha}$ B-crystallin (left) and tubulin (middle). Insets show an enlargement (threefold) of the boxed area in the right-hand panels. (C) Co-precipitations of ${alpha}$ B-crystallin and reconstituted MTs from L6E9 cell lysate. SDS-PAGE of total proteins (lane 1), the supernatant (lane 2), the precipitate (lane 3) in I and their immunoblots with the C1 antibody in II. Scale bars, 10 µm.