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Fig. 6. {alpha}B-Crystallin increases MT resistance to disassembly in vitro. (A) Changes of turbidity in assembly of MT proteins, over 15 minutes and disassembly after the addition of 10 µM podophyllotoxin (indicated by arrow) with (2.5 µM, 10 µM) or without {alpha}B-crystallin. (B) Mean levels of remaining MTs and standard deviations for each of three trials calculated from the ratio of the absorbance at 44-45 minutes to the level at 14-15 minutes (at polymerized peak) with different levels of {alpha}B-crystallin. {alpha}B-Crystallin (10 µM) significantly increased the amount of remaining MT compared with that in the absence of {alpha}B-crystallin. Values are means±s.d. (n=3, *significantly different at P<0.05). (C) Changes of turbidity in assembly of MT proteins over 15 minutes and disassembly after the addition of 1 mM calcium (indicated by arrow) with (2.5 µM, 5.0 µM, 10.0 µM) or without {alpha}B-crystallin. (D) The ratio of the attained polymerization as a mean value at 14-15 minutes after 113 µM calcium addition with {alpha}B-crystallin (10 µM) was significantly higher than that without {alpha}B-crystallin. Values are means±s.d. (n=6, **significantly different at P<0.01).