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Fig. 2. WtIGFBP-5 but not mutIGFBP-5 inhibits myogenesis. (A) Haematoxylin and Eosin staining of C2 cells transfected with control vector, wtIGFBP-5 (wtBP5) or mutIGFBP-5 (mutBP5), 72 hours after transfer to DM (5x magnification). (B; upper panel) Fluorescent immunocytochemistry to show myosin heavy chain (MHC) protein in myoblasts transfected as in A; (lower panel) DAPI staining of nuclei (40x magnification).