Fig. 6. WtIGFBP-5 and mutIGFBP-5 prevent the decrease in myoblast number induced by expression of antisense Igf2. Enriched myoblast populations expressing antisense Igf2 or vector (V) were additionally transfected with wtIGFBP-5 (wtBP5) or mutIGFBP-5 (mutBP5) or their control vector (C). Cell number is expressed as a percentage of values at the time of transfer from GM to DM for myoblasts at (A) 24 hours and (B) 48 and 72 hours in DM; means±s.e.m., n=3; ^**P<0.01 compared with V.