Fig. 7. WtIGFBP-5 and mutIGFBP-5 ameliorates the increased apoptosis induced by antisense Igf2 expression in myoblasts. Enriched myoblast populations expressing antisense Igf2 or vector (V) were additionally transfected with wtIGFBP-5 (wtBP5) or mutIGFBP-5 (mutBP5) or their control vector (C). (A) Flow cytometric determination of the percentage of annexin V-positive, PI-negative myoblasts after 48 hours in DM. (B) Caspase-3 activity in lysates derived from myoblasts transfected as in A. For A and B, data are expressed as a fraction of the values obtained for myoblasts transfected with antisense Igf2 alone; means±s.e.m., n=3; significance that mean is less than 1.0: ^*P<0.05; ^**P<0.01. ^***P<0.001.