(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 2. Physical interaction of endogenous IIGP and mHk3. Immunoprecipitation of endogenous IIGP or mHk3 from lysates of IFN{gamma}-stimulated or non-stimulated BMM. (A) The lysates were pre-cleared by immunoprecipitation with isotype matched control mAbs. Subsequently, IIGP was immunoprecipitated with the anti-IIGP mAb 5D9 either prior or after incubation for 30 min at 32°C with addition of GTP{gamma}S or GDPßS. Purified proteins were subjected to SDS-PAGE and western blotting. Co-immunoprecipitated mHk3 was revealed with an affinity-purified rabbit anti-hook3 serum. (B) The lysates were pre-cleared by use of a non-immune rabbit serum and mHk3 was subsequently purified from lysates of BMM stimulated with IFN{gamma} or not. IIGP was revealed on western blots using the anti-IIGP mAb 5D9. To determine the total amount of IIGP (B) or mHk3 (A) recovered by specific immunoprecipitation, 1/5 of the samples that were probed for co-purification were analyzed in parallel by western blotting with the respective antibodies.