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Fig. 3. Subcellular localization of IIGP and mHk3 in IFN{gamma}-stimulated BMM. The cells were stained with the anti-IIGP mAb 5D9 revealing the typical distribution of IIGP, which associates with the ER and the Golgi (A,D). The localization of mHk3 was revealed by employing an affinity-purified rabbit anti-hook3 serum (B,E). Cy2- (mHk3) and Cy3- (IIGP) labeled secondary reagents were used. In murine BMM mHk3 strongly accumulated in a juxtanuclear region reminiscent of the position of the MTOC and Golgi. In addition, a reticulate staining for mHk3 was observed in the perinuclear region. Close spatial proximity of IIGP and mHk3, which partially colocalized, was evident in the merged images (C,F).