Fig. 6. Localization of Mad2 and Bub1 checkpoint proteins is not affected by kinetochore dephosphorylation. S2 cells were lysed with detergent either in the presence (T+Mc) or in the absence (T-Mc) of microcystin, fixed and immunostained. (A) Immunostaining with the anti-Mad2 (green) and 3F3/2 (red) antibodies shows that Mad2 labelling remains unchanged after dephosphorylation. (B) Immunostaining with the anti-Bub1 (green) and 3F3/2 (red) antibodies shows that Bub1 still localizes normally after dephosphorylation. Scale bar: 5 µm.