Fig. 10. COS-1 cells transfected with Ext/R4Tth were labeled with [³³P]-phosphate for 1 hour in the absence or presence of the protein kinase inhibitors BIM, H-89 or staurosporine (St) as indicated. Inhibitors were used at micromolar concentrations as indicated. The cells were solubilized and the receptor was immunoprecipitated with anti-FGFR4 antibodies and analysed by SDS-PAGE and autoradiography.