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Fig. 11. COS-1 cells transfected with {Delta}Ext-GFP/R4Tth were examined live by fluorescence microscopy. A region of the nucleus and a region of the cytoplasm was photobleached and the recovery of fluorescence was recorded by time-lapse confocal microscopy. (A,B) Two examples of recovery of nuclear spots after bleaching showing slow recovery of immobile nuclear spots (A) or faster movement of mobile spots from the juxtanuclear region into the nucleus (B).