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Fig. 9. Cytosolic membrane stacks in COS-1 cells transfected with {Delta}Ext/R4Tth. (A) The cytosolic membrane stacks were positive for PDI (small gold particles) and {Delta}Ext/R4Tth (large gold particles). (B) Cytosolic membrane stack showing its flattened dense structure (plastic-embedded material). (C) Invaginations into the nucleus (arrowheads) were positive for {Delta}Ext/R4Tth (immunogold labeling) and contained several layers of membrane. Nu, nucleus. Scale bars, 200 nm.