Fig. 1. Sequence motifs present in the charged C-terminal domain of HDACm. (A) DNA encoding the C-terminal 167 amino acid residues of HDACm was expressed as a fusion protein with glutathionine S-transferase (GST-V). The fusion site is indicated by an arrow and trypsin sensitive sites are indicated by arrowheads. Potential CK2 phosphorylation sites are boxed by dashed lines, with the phosphorylated residue indicated by an asterisk. Groups of basic (K+R) residues, which might contribute to a nuclear import signal (NLS), are boxed by solid lines. (B) The proposed bipartite NLS of HDACm is aligned with the reported NLS of the histone H3/H4 chaperone N1/N2. Numbers indicate the residue positions in the protein sequences.