(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 3. Mapping of phosphorylation sites in HDACm. GST-{Delta}V was phospholabeled in vitro using oocyte nuclear CK2. (A) Only two phospholabeled tryptic peptides (1 and 2) were recovered by HPLC. (B) Sequencing of peptide 1 reveals two phospholabeled residues at positions S421 and S423. (C) Sequencing of peptide 2 reveals a single phospholabeled residue at positions S393. The potential site S388 (asterisk) is not labeled.