Fig. 3. The C-terminal region of pol protein is required for foci formation. MRC5 cells were transfected with plasmids expressing various eGFP-tagged pol deletion proteins and incubated for 20 hours. Cells were then UV irradiated with 10 J/m² or 10 mM HU and further incubated for 6 hours. (A) Summary of foci formation and cellular localisation properties of C-terminal truncation mutants. (B) Summary of foci formation and cellular localisation properties of N-terminal truncation mutants. C, cytoplasmic; C₂HC, C₂HC type Zinc finger domain; N, nuclear; NLS, nuclear localisation signal like domain; PC, similar to PCNA binding domain consensus; *1, both nuclear and cytoplasmic localisation, but majority was nuclear; *2, both nuclear and cytoplasmic localisation. (CI) Typical images of UV-irradiated cells expressing eGFP-tagged pol deletion proteins.