Fig. 10. Bgs4p localizes to the different sites of cell wall growth during spore germination. The homothallic GFP-bgs4⁺ bgs4 h⁹⁰ strain was grown and sporulated for 7 days as in Fig. 8. The spores were collected and incubated in YES liquid medium at 28°C. Samples were taken at 3, 7, 9 and 11 hours, and examined as in Fig. 4A. A total of 252 spores from 42 different images were ordered and analyzed. Spores illustrative of each germination step were selected and ordered to show a germination progression. Bar, 2 µm.