Fig. 5. Bgs4p localization during septation depends on medial ring formation and positioning, but the SIN proteins may be dispensable for its localization to the medial ring and septum. GFP-bgs4⁺ bgs4 mutant cells were grown as in Fig. 4A, shifted to 32°C (cdc3-6, cdc15-140 and cdc16-116) for 4 hours or to 37°C (mid1-366, cdc14-118) for 3-6 hours, and visualized as in Fig. 4A, except cdc14-118 cells, which were also analyzed for nucleus staining as in Fig. 4C. Cells representative of the different mutant phenotypes are shown. In mid1-366, cells with overlapping and non-overlapping CW (arrowhead) and GFP-Bgs4p (arrow) fluorescence are shown. In cdc14-118, cells representative of the phenotype at different times are shown, including cells displaying a partial septum (arrowhead) synthesized before they expressed the mutant phenotype, some of them with GFP-Bgs4p (arrow) localized to the partial septum structure. Bar, 2 µm.