Fig. 6. Bgs4p localization depends on polarity establishment proteins and on the actin cytoskeleton. (A) Bgs4p localizes to the altered growing poles and septa in the end-marker mutants tea1-1, tea2-1 and tea2-1 cdc11-119. (B) Bgs4p localizes all around the cell membrane and septum in the actin mutant cps8-188. GFP-bgs4⁺ bgs4 mutant cells were grown as in Fig. 4A, shifted to 37°C for 6 hours (cps8-188 and tea2-1 cdc11-119) or 8 hours (tea1-1 and tea2-1), and visualized as in Fig. 4A. Bar, 2 µm.