Fig. 8. Bgs4p is present and localized to all sites of cell wall synthesis during sexual differentiation. Homothallic GFP-bgs4⁺ bgs4 h⁹⁰ cells grown on EMM at 28°C up to the early stationary phase were transferred onto SPA plates and incubated at 28°C. Samples were collected at 3, 5, 8, 24 and 48 hours, resuspended in EMM medium containing CW (50 µg/ml), and visualized as in Fig. 4A. A total of 435 mating cells and zygotes from 86 different images were ordered and analyzed. Cells and zygotes representative of each mating and sporulation step were selected and ordered to show a sexual phase progression. Bar, 2 µm.