Fig. 5. CAP350 does not affect RXR or TR subcellular localization. Representative images of NIH3T3 cells transfected with an expression vector for RXR alone (A-C) or TR alone (J-L) or cotransfected with expression vectors for RXR and CAP350 (D-F) or TR and CAP350 (M-O), and analyzed by indirect immunofluoresence microscopy with anti-RXR antibody or anti-TR antibody (green) and anti-c-Myc antibody (red) to detect tagged CAP350. The merged images indicate that CAP350 does not alter the subcellular localization of RXR or TR (F and O, respectively). Nuclei were detected by staining with Hoechst (B and K). (G-I) PPAR ligand does not affect the subcellular relocalization of PPAR in the presence of CAP350. NIH3T3 cells were transfected with expression vectors for PPAR and CAP350 in the presence of the PPAR ligand Panagonist B and subjected to immunostaining with anti-PPAR antibody (green) and anti-c-Myc antibody (red) to detect tagged CAP350. The merged image (I) shows that ligand does not alter the colocalization of PPAR and CAP350. Bars, 10 µm.