Fig. 6. CAP350 colocalizes with the centrosome. (A) NIH3T3 cells were cotransfected with expression plasmids for CAP350 and ECFP-Golgi in the absence (panels A-C) or presence (panels D-F) of Brefeldin A. CAP350 was detected by immunostaining with anti-c-Myc antibody (red). The merged images show that CAP350 does not colocalize with the Golgi complex. (B) CAP350 is present in the centrosome. NIH3T3 cells were transfected with the expression plasmid for CAP350 and subjected to immunostaining with anti-pericentrin antibody (green) (panel A) and anti-c-Myc antibody (red) to detect tagged CAP350 (panel B). The merged image indicates colocalization of CAP350 with pericentrin in the centrosome, as shown by the yellow color. (See also Movie 3 in supplementary material.) (C) CAP350 colocalizes with -tubulin. NIH3T3 cells were cotransfected with plasmids expressing EYFP--tubulin (panel A) and CAP350 (panel B). CAP350 was detected by immunostaining with anti-c-Myc antibody (red). The colocalization of CAP350 with EYFP--tubulin is seen as yellow in the merged image (panel C). Bars, 10 µm.