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Fig. 7. CAP350 colocalizes with intermediate filaments (IF). (A) NIH3T3 cells were transfected with expression plasmid for CAP350 and subjected to immunostaining with anti-cytokeratin antibody (green; panel A) and anti-c-Myc antibody (red) to detect tagged CAP350 (panel B). The merged image (panel C) shows colocalization of CAP350 and cytokeratin, a marker of IFs, as indicated by the yellow color. (B) CAP350 does not colocalize with mitochondria. NIH3T3 cells were cotransfected with expression plasmids for ECFP-Mito (panel A) and CAP350 (panel B). CAP350 was detected by immunostaining with anti-c-Myc antibody (red). The merged image (panel C) shows that CAP350 does not colocalize with mitochondria. (C) CAP350 does not colocalize with actin filaments. NIH3T3 cells transfected with expression plasmid for CAP350 were analyzed by staining with phalloidin-FITC to detect actin (green) (panel A) and anti-c-Myc antibody (red) to detect CAP350 (panel E). The merged image (panel C) shows that CAP350 does not colocalize with actin filaments. Bars, 10 µm.