JCS -- Patel et al. 118 (1): 175 Figure IG8

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Fig. 8. CAP350 and PPAR ${alpha}$ colocalize to novel subnuclear foci. NIH3T3 cells were cotransfected with expression plasmids for CAP350 and GFP-SF2/ASF (A-D), GFP-NUP98 (E-H), or GFP-PML (I-K), as indicated. CAP350 was detected by immunostaining with anti-c-Myc antibody (red). Nuclei were detected by staining with Hoechst. The respective merged images indicate that the CAP350-containing subnuclear foci do not represent speckles, Nup98 bodies or PML bodies. Formation of CAP350-PPAR ${alpha}$ subnuclear foci does not require active transcription. NIH3T3 cells were cotransfected with plasmids expressing CAP350 and PPAR ${alpha}$ in the presence of the transcriptional inhibitor, actinomycin D (M-P). CAP350 and PPAR ${alpha}$ were detected by immunostaining with anti-c-Myc antibody (red) and anti-PPAR ${alpha}$ antibody (green), respectively. Nuclei were detected by staining with Hoechst. The merged image (P) indicates that the colocalization of CAP350 and PPAR ${alpha}$ in subnuclear bodies does not require ongoing RNA synthesis. Bars, 10 µm.