Fig. 5. Localization of Hob1, but not Wsp1, depends on F-actin integrity. (A) IRG2 (hob1-GFP) cells (Hob1-GFP and actin panels) or SPU (wild type) cells expressing GFP/-tubulin (microtubules) were grown to mid-log phase and incubated for 15 minutes in the presence of 1% dimethyl sulfoxide (DMSO) (top), 20 µM LatA and 1% DMSO (LatA, middle) or 100 µM TBZ and 1% DMSO (TBZ, bottom). Some cells were then fixed and stained with TRITC-phalloidin to observe actin localization. (B) Cells expressing Wsp1-GFP were similarly grown to mid-log phase, treated with DMSO, LatA or TBZ, and examined for Wsp1-GFP localization.