Fig. 2. PARP-1 and PARP-2 accumulate in the nucleolus independently of each other. Wide-field views of the simultaneous immunodetection of PARP-2 and PARP-1 in wild type (top), PARP-1^–/– (middle) and PARP-2^–/– (bottom) MEFs fixed with 1% formaldehyde/0.1% Triton X-100. DNA is stained with DAPI. Co-immunodetection of PARP-2 and B23 was performed in parallel under the same conditions, only the B23 staining is shown in the right column. Bar, 10 µm.