Fig. 3. Localization in living PARP-2^–/– MEFs of full-length and truncated versions of mouse PARP-2 fused to the C-terminus of GFP. (Top) Schematic representation of mPARP-2 or truncated versions of mPARP-2 fused to GFP. When indicated, the NLS of SV40-T was inserted in frame between the coding sequence of GFP and the sequence from mPARP-2. Fusion proteins were present (+) or not (–) in the nucleus or the nucleolus. (Bottom) Localization in living PARP-2^–/– MEFs of the GFP-fused recombinant proteins (left panels) labelled as in the scheme above. The corresponding DIC images are shown on the right. Bar, 10 µm.