Fig. 7. PARP-1 and PARP-2 are only moderately delocalized from the nucleolus upon alkylated or oxidative DNA damage. (A) Wide field views of the immunodetection of PARP-2 and PARP-1 in wild-type MEFs treated or not (NT, not treated) with H₂O₂ (0.2 or 2 mM for 2 hours), MMS (0.2 or 2 mM for 2 hours) or 1 µg/ml 4NQO for 1 hour. Cells were fixed with 1% formaldehyde/0.1% Triton X-100. DNA is stained with DAPI. Co-immunodetection of PARP-2 and B23 in MEF cells treated in the same conditions. Only the B23 staining is shown in the panels on the right. (B) Western blot analyses with anti-PARP-1 or anti-PARP-2 antibodies performed on crude extracts of 2.5x10⁵ MEFs submitted to the indicated treatments or not treated (–). Bar, 10 µm.