Fig. 7. nhr-25 RNAi disrupts formation of the adult alae and the lateral epidermis. (A,B) Transmission electron microscopy sections reveal aberrant cuticle with shallow alae (arrows) in nhr-25 RNAi adults (B). The lateral hyp7 syncytium (h) has an abnormal structure, with much of the dense membrane system missing. The dark material (B) is yolk not taken up by the gonad. (C,D) Scanning electron micrographs of wild-type (C) and nhr-25(RNAi) (D) adults viewed from the lateral side. Alae are shown with arrows, bracket (D) indicates a lateral region of sparse annuli. (E) A gap in the alae (delimited by arrowheads) caused by nhr-25 RNAi. (F,G) Branching of the adult alae caused by a dominant mutation of the cuticle gene rol-6 in the SU93 strain (F) is enhanced by nhr-25 RNAi (G); a severe case is shown, corresponding to `>1 small loops' in Table 1. The underlying seam adherens junctions, marked by expression of the ajm-1::gfp reporter (F') mirrors the shape of the alae. Arrowheads indicate the branching points. Bars, 2 µm (A,B), 10 µm (C,D), 20 µm (E-G).