Fig. 2. Ataxin-1 nuclear inclusion formation and movement are not dependent on polyglutamine. (A-C) Fluorescence microscopy images of eGFP ataxin-1[Q2] (A), [Q26] (B) or [Q84] (C) from live-cell video observations at 37°C in HeLa cells. (D-F) Three dimensional x,y,t voxel and isosurface representation of ataxin-1 body movement in either Q2 (D), Q26 (E), or Q84 (F) context showing similar body movement. Bar: 10 µm. (See also Movies 3-5 in supplementary material.) D-F are presented as red-cyan stereo images.