JCS -- Irwin et al. 118 (1): 233 Figure IG7

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Fig. 7. Wild-type ataxin-1 can shuttle to and from the nucleus. FRAP live cell shuttle assay. Laser confocal fluorescence over DIC overlay of either GFP-wild-type or -mutant ataxin-1 proteins in bikaryons treated with cycloheximide before (panels A,F) and after (panels C,H) photo bleaching the entire area of the bikaryon except one nucleus (mask area, panels B, G). Recovery of wild-type ataxin-1 is seen by 10 minutes (D,E), but no recovery of mutant ataxin-1 is seen even after 30 minutes (I,J). Bar: $~$ 10 µm.