Fig. 10. Immunolocalization of HS in C2.7 myoblasts. Localization of HS subspecies was investigated using (A) RB4CD12, (B) AO4F12 and (C-E) AO4BO5 antibodies and confocal microscopy. Note the differential labelling of HS species either at the periphery of cells (RB4CD12) or at cell-to-cell contacts with AO4BO5 or AO4F12. These two antibodies labelled cell-to-cell contacts differentially. Areas indicated by arrows are described in the text. Panel E shows the boxed area in C. Bar, 10 µm.