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Fig. 9. ELISA assay of HS species extracted from differentiating C2.7 cells. GAG samples were prepared from differentiating controls and RGTA-treated cells of one representative culture. They were grafted on BSA and tested in an ELISA assay using different HS antibodies (RB4CD12, AO4BO5 and AO4F12). Each value is the mean of duplicate measurements.