Fig. 6. The cortical ER tubular network is defective in the ice2 mutant. Wild-type (strain SFNY1061) (A), ice2 mutant (strain SFNY1282) (B) and sec3 mutant (strain SFNY1449) (C) cells expressing the ER marker Hmg1p-GFP were analysed using fluorescence microscopy. Images were obtained from the centre of the cell and from the cell periphery. Arrowheads point to a continuous network displayed in the mother cell (A,C). Arrows point to buds that display a normal cortical ER network (A) and buds that lack an intact cortical ER network (B). An abnormal ER network is observed in the mother cell of the ice2 mutant (B). (D) Quantitation of wild-type and mutant cells displaying an aberrant cortical ER network.