Fig. 8. The expression of TbMBAP1 is developmentally regulated. (A) Immunoblot showing the decrease in TbMBAP1 during development from the bloodstream stage (BSF) to the procyclic insect stage (PCF). Developmental transformation was induced in vitro by the addition of cis-aconitate and a change in culture temperature from 37°C to 27°C. Note that the downregulation of TbMBAP1 is accompanied by a marked mobility-shift of the protein after 72 hours. TbHSP60 was used as a loading control. (B) Relative TbMBAP1 activity at various time points during differentiation. (C) Kinetics of endocytosis of the fluid-phase marker Alexa Fluor^TM 488 dextran during development to the procyclic insect stage. At the time points indicated, cells were incubated with fluorescent dextran for 15 minutes at 27°C. Internalization is expressed as the change in relative fluorescence intensity/cell.