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Fig. 2. Actin dynamics are linked to respiratory function. (A) Wild-type, {Delta}end3 and sla1{Delta}118-511 cells were plated on media containing either glucose or the non-fermentable carbon source glycerol. Wild-type cells grew on both media, whereas both {Delta}end3 and sla1{Delta}118-511 cells were unable to grow on glycerol, indicating that these cells have a respiratory defect. (B,C) Mitochondrial morphology in log phase (B) and stationary phase (C) wild-type, {Delta}end and sla1{Delta}118-511 cells was assessed using a targeted GFP molecule. (D-F) Mitochondrial membranes were visualized by DiOC6 staining in wild-type (D), {Delta}end3 (E) and sla1{Delta}118-511 (F) cells at log phase. Bars, 10 µm.