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Fig. 5. Actin-stabilized end3-1 cells display markers of apoptosis. ROS accumulation using H2DCFDA (A) and caspase activation using FITC–VAD-fmk (B) were measured by flow cytometry in wild-type and end3-1 cells grown to log phase at 24°C and after 2 hours of growth at 37°C. (C) The viabilities were calculated of wild-type and end3-1 cultures grown to log phase at 24°C and after a shift to 37°C for 2 hours.