JCS -- Gourlay and Ayscough 118 (10): 2119 Figure IG6

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Fig. 6. Overexpression of RSP5 can improve mitochondrial function, increase viability and restore actin dynamics in ${Delta}$ end3 cells. (A) Wild-type, ${Delta}$ end3 and ${Delta}$ end3[RSP5] cells were plated onto YP agar containing 2% glucose or 3% glycerol as the sole carbon source and grown for 3 days at 37°C. (B) Cultures from each of these strains were then assayed for ROS accumulation by flow cytometry using H₂DCFDA. (C) Cell viability was assessed via a plating assay carried out in triplicate. (D) Wild-type, ${Delta}$ end3 and ${Delta}$ end3[RSP5] cells were grown to stationary phase and stained for F-actin with rhodamine-phalloidin. (E) The same strains were assayed for glycogen accumulation by iodine staining. Bar, 10 µm.