JCS -- Gourlay and Ayscough 118 (10): 2119 Figure IG8

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Fig. 8. PDE2 overexpression reduces ROS accumulation in ${Delta}$ end3 cells and restores viability. (A) Wild-type and ${Delta}$ end3 cells carrying either an empty Yep13 plasmid or Yep13 containing PDE2 (Yep13[PDE2]) were struck onto YPD plates containing 0 mM or 3 mM H₂O₂ and grown for 3 days at 30°C. (B) ROS accumulation was assessed in wild-type cultures containing YEp13, ${Delta}$ end3 cultures containing YEp13 and ${Delta}$ end3 cultures containing Yep13[PDE2] that had been grown overnight in YPD at 30°C. Cells were visualized after staining by ultraviolet fluorescence microscopy. (C) The proportion of viable cells was assessed in overnight wild-type cultures containing YEp13, wild-type cultures containing YEp13[PDE2], ${Delta}$ end3 cultures containing YEp13 and ${Delta}$ end3 cultures containing YEp13[PDE2] cultures. Bar, 10 µm.