Fig. 1. Expression of YFP-tagged Kv channels in HEK cells. Confocal images of YFP-Kv2.1HA (A), YFP-Kv1.4myc (B) and YFP-Kv1.3 (C). Surface channels were detected by incubation of live cells with either anti-HA (to detect Kv2.1HA) or anti-myc (to detect Kv1.4myc) antibodies before fixation and Alexa 594-labeled secondary antibody addition as described in Materials and Methods. Colour coding is as follows: red, immunofluorescence surface labeling of the extracellular HA or myc epitopes; green, YFP fluorescence. YFP-Kv1.3 was not epitope tagged, therefore only YFP fluorescence is shown. Optical sections correspond to the relative middle of each cell. Bars, 10 µm.