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Fig. 2. CFP-Kv2.1HA and YFP-Kv1.4myc do not colocalize when co-expressed. Confocal image of a live HEK cell expressing both CFP-Kv2.1HA and YFP-Kv1.4myc. (A) The CFP fluorescence, (B) the YFP and (C) the overlay of the CFP and YFP signals. CFP is pseudocolored green and YFP is pseudocolored red. (D) Plot of CFP and YFP pixel intensity verses cell surface length within the boxed region shown in C. Note that while both channels are expressed in the same regions, the intensity profiles indicate that any true colocalization is unlikely to occur.