Fig. 8. YFP-Kv2.1 and YFP-Kv1.4 sediment differently on a sucrose density gradient. Whole-cell homogenates from HEK cells expressing either YFP-Kv2.1HA (top) or YFP-Kv1.4myc (bottom) were sedimented through a 5-20% sucrose density gradient (see Materials and Methods) to estimate channel-containing complex size. Kv2.1 and Kv1.4-containing gradient fractions were identified via western blot analysis using anti-GFP antibody. Thyroglobulin (660 kDa) was used as a size standard, giving a symmetrical peak in fraction 4. P represents the pellet material collected from the bottom of the tube.