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Fig. 4. Disruption of the Cx43/ZO-1 interaction reduces gap junctional communication in transfected ROS cells. Lucifer yellow was injected into single cells in confluent monolayers, and the number of dye filled cells was scored after 3 minutes (N=25). Dye transfer was significantly reduced in ROS/ZO- and clones E, J, and K compared to parental ROS cells. Results are expressed as the mean number of dye filled cells per injected cell ± s.d.