Fig. 2. Accumulation of focal contact marker proteins in the vicinity of attached S. aureus. (A) 293 cells were transfected with GFP-tensin and infected for 1 hour with rhodamine-labelled S. aureus or S. carnosus at a MOI 20. After fixation, samples were examined by confocal microscopy. Cell-associated S. aureus (arrows) or S. carnosus (arrowheads) are indicated. Bars, 10 µm. (B) Local recruitment of GFP-tensin (green line) to cell-associated bacteria (red line) was quantified by plotting the fluorescence intensity as detected in the GFP or rhodamine channels, respectively, against the distance. (C) 293 cells were transfected with GFP-zyxin, infected and processed as in A. Bars, 10µm.