Fig. 3. Interference with FAK function blocks uptake of pathogenic S. aureus. (A) 293 cells were transfected with HA-FAK K454M, HA-FAK Y397F, HA-FAK Pro(–), or the empty expression vector (pcDNA). After 2 days, cells were infected with S. aureus for 2 hours at MOI of 20 and the number of internalised bacteria was determined by gentamicin/lysostaphin protection assays. Values are means±s.d. of three independent experiments done in triplicate. (Lower panel) Western blotting of whole cell lysates (WCL) with anti-HA-tag antibody shows the expression of HA-tagged FAK constructs. (B) 293 cells were transfected with GFP-FRNK or the empty expression vector (pcDNA) and used as in A. Values are means±s.d. of two independent experiments done in triplicate. (Lower panel) WCLs were probed with anti-GFP antibody.