Fig. 1. ARF6 recruitment and distribution during bacterial entry. Cells transfected with HA-tagged-ARF6 WT were infected for 5 minutes with FITC-coupled C. caviae GPIC (white spots). Cells were fixed and stained with anti-HA antibody and Cy5-labelled secondary antibody (top panel) and with Alexa 546-coupled phalloidin (middle panel). The bottom panel is the merged image of ARF6 (red), actin (green) and bacteria (white) staining. A medial confocal section is shown. x-z and y-z optical sections on the positions marked by the crossed lines are displayed on the top and right of the image. ARF6 recruitment is observed at a site of intense actin polymerization, where a bacterium is present (arrows). Bar, 5 µm.