Fig. 3. Degranulation in complexin II knockdown cells. (A) Wild-type or knockdown cells were sensitized with IgE and stimulated with antigen (100 ng/ml DNP₆-BSA). The quantity of ß-hexosaminidase in the supernatant is expressed as a percentage of total ß-hexosaminidase. Values were obtained from four independent preparations of wild-type cells () and four independent knockdown clones () [means.e.m. (n=4)]. (B) Cells were stimulated with A23187 (1 µM) and PMA (50 ng/ml). Values were obtained and plotted as mentioned above.